Mammalian cell culture media HCF

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Fibroblast Growth Medium

PromoCell

Upstream tips	Protocol tips	Downstream tips
	To mimic effects of inflammation, cells were incubated with LPS 10 ng/ml and control cells incubated without LPS, for 24 h.

Protocol tips
To mimic effects of inflammation, cells were incubated with LPS 10 ng/ml and control cells incubated without LPS, for 24 h.

Manufacturer protocol Publication protocol 1 Relevant paper

FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit

Lonza

Upstream tips	Protocol tips	Downstream tips
	cultured in FGM-3 medium, in a humidified incubator at 90% N2, 5% O2, 5% CO2 at all times unless otherwise indicated

Protocol tips
cultured in FGM-3 medium, in a humidified incubator at 90% N2, 5% O2, 5% CO2 at all times unless otherwise indicated

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM/F-12

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	DMEM:F12 supplemented with 10% human serum, penicillin/streptomycin

Protocol tips
DMEM:F12 supplemented with 10% human serum, penicillin/streptomycin

Manufacturer protocol Publication protocol 1 Relevant paper

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