Mammalian cell culture media HFLS

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Human Synoviocyte Growth Medium

Cell Applications Inc

Upstream tips	Protocol tips	Downstream tips
	Synovial tissues were cut into small pieces and digested with collagenase (Sigma-Aldrich, St Louis, MO) in Dulbecco’s modified Eagle’s medium (DMEM) for 2 h at 37 °C to isolate FLS. The FLS were grown in DMEM containing 10 % FetalClone 1 (Thermo Scientific, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin (Sigma-Aldrich, St Louis, MO) in a humidified incubator at 37 °C under 5 % CO2. At confluence, the cells were trypsinised and passaged; all cells were used in experiments between passages 3 and 8.

Protocol tips
Synovial tissues were cut into small pieces and digested with collagenase (Sigma-Aldrich, St Louis, MO) in Dulbecco’s modified Eagle’s medium (DMEM) for 2 h at 37 °C to isolate FLS. The FLS were grown in DMEM containing 10 % FetalClone 1 (Thermo Scientific, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin (Sigma-Aldrich, St Louis, MO) in a humidified incubator at 37 °C under 5 % CO2. At confluence, the cells were trypsinised and passaged; all cells were used in experiments between passages 3 and 8.

Protocol tips

Synovial tissues were cut into small pieces and digested with collagenase (Sigma-Aldrich, St Louis, MO) in Dulbecco’s modified Eagle’s medium (DMEM) for 2 h at 37 °C to isolate FLS. The FLS were grown in DMEM containing 10 % FetalClone 1 (Thermo Scientific, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin (Sigma-Aldrich, St Louis, MO) in a humidified incubator at 37 °C under 5 % CO2. At confluence, the cells were trypsinised and passaged; all cells were used in experiments between passages 3 and 8.

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM

PAN biotech

Upstream tips	Protocol tips	Downstream tips
	FLS were cultured in a humidified 10% CO2 atmosphere at 37 °C using DMEM medium supplemented with 1.0 g/L glucose, 584 mg/L l-glutamine, 10% fetal bovine serum (FBS), 10 mM HEPES buffer, 10 U/mL penicillin, and 0.1 mg/mL streptomycin. The experiments were performed with cells from passage No. 5. Routine tests for mycoplasma contamination using the PCR Mycoplasma Kit (PromoCell, Heidelberg, Germany) were negative.

Protocol tips
FLS were cultured in a humidified 10% CO2 atmosphere at 37 °C using DMEM medium supplemented with 1.0 g/L glucose, 584 mg/L l-glutamine, 10% fetal bovine serum (FBS), 10 mM HEPES buffer, 10 U/mL penicillin, and 0.1 mg/mL streptomycin. The experiments were performed with cells from passage No. 5. Routine tests for mycoplasma contamination using the PCR Mycoplasma Kit (PromoCell, Heidelberg, Germany) were negative.

Protocol tips

FLS were cultured in a humidified 10% CO2 atmosphere at 37 °C using DMEM medium supplemented with 1.0 g/L glucose, 584 mg/L l-glutamine, 10% fetal bovine serum (FBS), 10 mM HEPES buffer, 10 U/mL penicillin, and 0.1 mg/mL streptomycin. The experiments were performed with cells from passage No. 5. Routine tests for mycoplasma contamination using the PCR Mycoplasma Kit (PromoCell, Heidelberg, Germany) were negative.

Manufacturer protocol Publication protocol 1 Relevant paper

Gibco™ DMEM, high glucose

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Synovial tissues were cut into small pieces and digested with collagenase (Sigma-Aldrich, St Louis, MO) in Dulbecco’s modified Eagle’s medium (DMEM) for 2 h at 37 °C to isolate FLS. The FLS were grown in DMEM containing 10 % FetalClone 1 (Thermo Scientific, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin (Sigma-Aldrich, St Louis, MO) in a humidified incubator at 37 °C under 5 % CO2.

Protocol tips
Synovial tissues were cut into small pieces and digested with collagenase (Sigma-Aldrich, St Louis, MO) in Dulbecco’s modified Eagle’s medium (DMEM) for 2 h at 37 °C to isolate FLS. The FLS were grown in DMEM containing 10 % FetalClone 1 (Thermo Scientific, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin (Sigma-Aldrich, St Louis, MO) in a humidified incubator at 37 °C under 5 % CO2.

Manufacturer protocol Publication protocol 1 Relevant paper

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