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Found 2 matching solutions for this experiment
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supplemented with 10% fetal bovine serum (FBS) and 1% mix of antibiotics/antifungals (Sigma-Aldrich, US) in polystyrene cell culture flasks (Corning, US). The cultures were kept at 37 °C in a humidified atmosphere at 5% CO2 and removed from the flask with trypsin (Trypsin-EDTA solution; Sigma-Aldrich, US). |
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supplemented with 10% fetal bovine serum (FBS) and 1% mix of antibiotics/antifungals (Sigma-Aldrich, US) in polystyrene cell culture flasks (Corning, US). The cultures were kept at 37 °C in a humidified atmosphere at 5% CO2 and removed from the flask with trypsin (Trypsin-EDTA solution; Sigma-Aldrich, US). |
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Human umbilical vein endothelial cells (HUVECs, Lonza, CC-2517A) were suspended in the recommended cell culture medium (basal media with growth factors, Lonza, CC-3162). |
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Human umbilical vein endothelial cells (HUVECs, Lonza, CC-2517A) were suspended in the recommended cell culture medium (basal media with growth factors, Lonza, CC-3162). |
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