Mammalian cell culture media MA-104

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Protocol tips
supplemented with 10% fetal bovine serum (ThermoFisher Scientific), 1% penicillin/streptomycin, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate in a 5% CO2 atmosphere at 37°C.
Protocol tips
supplemented with 5% fetal bovine serum (Hyclone, Logan, UT, U.S.A.) and antibiotics (100 units/ml of penicillin and 100 µg/ml of streptomycin).
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