Mammalian cell culture media MCF-10A

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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DMEM/F-12

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	MCF-10A cells were cultured as described []. VSV-G pseudotyped retroviruses were generated, and MCF-10A cells were infected and selected as described [] using a VSV-GPG producer line []. VSV-G pseudotyped lentiviruses were produced by co-transfection of pLKO hairpin vectors with accessory plasmids in 293T cells. Hairpin-encoding lentiviruses were used to infect MCF-10A cells or superinfect CRB3-expressing cells, and stable cell lines were obtained by selection with puromycin (2 μg/ml) or dual selection with puromycin (2 μg/ml) and G418 (300 μg/ml), respectively.

Protocol tips
MCF-10A cells were cultured as described []. VSV-G pseudotyped retroviruses were generated, and MCF-10A cells were infected and selected as described [] using a VSV-GPG producer line []. VSV-G pseudotyped lentiviruses were produced by co-transfection of pLKO hairpin vectors with accessory plasmids in 293T cells. Hairpin-encoding lentiviruses were used to infect MCF-10A cells or superinfect CRB3-expressing cells, and stable cell lines were obtained by selection with puromycin (2 μg/ml) or dual selection with puromycin (2 μg/ml) and G418 (300 μg/ml), respectively.

Protocol tips

MCF-10A cells were cultured as described []. VSV-G pseudotyped retroviruses were generated, and MCF-10A cells were infected and selected as described [] using a VSV-GPG producer line []. VSV-G pseudotyped lentiviruses were produced by co-transfection of pLKO hairpin vectors with accessory plasmids in 293T cells. Hairpin-encoding lentiviruses were used to infect MCF-10A cells or superinfect CRB3-expressing cells, and stable cell lines were obtained by selection with puromycin (2 μg/ml) or dual selection with puromycin (2 μg/ml) and G418 (300 μg/ml), respectively.

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM–Dulbecco's Modified Eagle Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS; HyClone; GE Healthcare Life Sciences, Logan, UT, USA

Protocol tips
supplemented with 10% fetal bovine serum (FBS; HyClone; GE Healthcare Life Sciences, Logan, UT, USA

Manufacturer protocol Publication protocol 1 Relevant paper

MEGM-Mammary-Epithelial-Cell-Growth-Medium

Lonza

Upstream tips	Protocol tips	Downstream tips
	supplemented with 100 ng/mL cholera toxin (Sigma, USA).

Protocol tips
supplemented with 100 ng/mL cholera toxin (Sigma, USA).

Manufacturer protocol Publication protocol 1 Relevant paper

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