Mammalian cell culture media MCF-7

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Protocol tips
All different cell lines were obtained from American Type Culture Collection (ATCC),
Manassas, VA, USA). Human adenocarcinoma breast cells MCF-7 were maintained in DMEM-F12
(Dulbecco's Modified Eagle's Medium (DME) and Ham's F-12 Nutrient Mixture) containing 5%
newborn calf serum (NCS), 1% glutamine, 1% penicillin/streptomycin, all from Sigma-Aldrich). MDAMB-231 and MDA-MB-468 TNBC cells were cultured in DMEM-F12 (Dulbecco's Modified Eagle's
Medium (DME) and Ham's F-12 Nutrient Mixture) containing 10% heat inactivated fetal bovine
serum (FBS), 1% glutamine, 1% penicillin/streptomycin, all from Sigma-Aldrich (Sigma–Aldrich).
Protocol tips
supplemented with 1% penicillin/strepto mycin (Sigma-Aldrich, St. Louis, MO, USA) and 10% FBS (HyClone)
Gibco™ RPMI 1640 Medium

Fisher Scientific

Protocol tips
supplemented with 10% fetal bovine serum (Gibco, USA).
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