Mammalian cell culture media RAOEC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Gibco™ DMEM, high glucose

Fisher Scientific

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	Rat aortic ECs were purchased from CELL applications, Inc. (San Diego, CA, USA). Rat aortic smooth muscle cells (VSMCs), line A10 (ATCC), were maintained in Dulbecco's Modified Eagle Medium (DMEM, GIBCO). Primary cultures of human coronary artery smooth muscle cells (HCASMCs) were obtained from Cascade Biologics™ (Life Technologies).

Protocol tips
Rat aortic ECs were purchased from CELL applications, Inc. (San Diego, CA, USA). Rat aortic smooth muscle cells (VSMCs), line A10 (ATCC), were maintained in Dulbecco's Modified Eagle Medium (DMEM, GIBCO). Primary cultures of human coronary artery smooth muscle cells (HCASMCs) were obtained from Cascade Biologics™ (Life Technologies).

Manufacturer protocol Publication protocol 1 Relevant paper

Rat Endothelial Cell Growth Medium

Cell Applications Inc

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	supplemented with 10% fetal bovine serum (FBS, Invitrogen, #10082147), 100 U/mL penicillin (Invitrogen, #15140122), 100 μg/mL streptomycin (Invitrogen, #15140122), and 4 mmol/L L‐glutamine (VWR, West Chester, PA, #45000‐676)

Protocol tips
supplemented with 10% fetal bovine serum (FBS, Invitrogen, #10082147), 100 U/mL penicillin (Invitrogen, #15140122), 100 μg/mL streptomycin (Invitrogen, #15140122), and 4 mmol/L L‐glutamine (VWR, West Chester, PA, #45000‐676)

Manufacturer protocol Publication protocol 1 Relevant paper

EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit

Lonza

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	Supplemented with 10% fetal bovine serum and EGM-2 SingleQuotes

Protocol tips
Supplemented with 10% fetal bovine serum and EGM-2 SingleQuotes

Manufacturer protocol Publication protocol 1 Relevant paper

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