Mammalian cell culture media U87

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

DULBECCO MEM MEDIUM

Vitrocell

Upstream tips	Protocol tips	Downstream tips
	cells were trypsinized and resuspended (16–32 × 106 cells/mL; >95% viability) in DMEM supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin (sDMEM) containing 20 mM HEPES.

Protocol tips
cells were trypsinized and resuspended (16–32 × 106 cells/mL; >95% viability) in DMEM supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin (sDMEM) containing 20 mM HEPES.

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate

Biowest

Upstream tips	Protocol tips	Downstream tips
	supplemented with fetal bovine serum 10% (FBS, Biowest, FRA), pyruvate (1 mM; InVitro SA, MEX), non-essential amino acids (0.1 mM; InVitro SA, MEX), and a mix of antibiotics (1 mM; InVitro SA, MEX)

Protocol tips
supplemented with fetal bovine serum 10% (FBS, Biowest, FRA), pyruvate (1 mM; InVitro SA, MEX), non-essential amino acids (0.1 mM; InVitro SA, MEX), and a mix of antibiotics (1 mM; InVitro SA, MEX)

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM–Dulbecco's Modified Eagle Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco)

Protocol tips
supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco)

Manufacturer protocol Publication protocol 1 Relevant paper

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