Mammalian cell culture media Vero

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Minimum Essential Media (MEM)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	minimal essential medium (MEM; Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% penicillin-streptomycin combination, 1% glutamine, and 2% NaHCO3 (pH 7.4) at 37 °C in a 5% CO2 atmosphere.

Protocol tips
minimal essential medium (MEM; Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% penicillin-streptomycin combination, 1% glutamine, and 2% NaHCO3 (pH 7.4) at 37 °C in a 5% CO2 atmosphere.

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM–Dulbecco's Modified Eagle Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (ThermoFisher Scientific), 1% penicillin/streptomycin, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate in a 5% CO2 atmosphere at 37°C.

Protocol tips
supplemented with 10% fetal bovine serum (ThermoFisher Scientific), 1% penicillin/streptomycin, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate in a 5% CO2 atmosphere at 37°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Minimum Essential Medium Eagle

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	enriched with 10% Fetal Bovine Serum (FBS) (Sigma Aldrich) and 2 mM l-glutamine (Sigma Aldrich)

Protocol tips
enriched with 10% Fetal Bovine Serum (FBS) (Sigma Aldrich) and 2 mM l-glutamine (Sigma Aldrich)

Manufacturer protocol Publication protocol 1 Relevant paper

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