Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

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Found 3 matching solutions for this experiment

3D-Gene® Mouse miRNA Oligo chip (ver.21)

Toray

Upstream tips	Protocol tips	Downstream tips
	Samples of CCl-induced liver tumors (a mixture of five independent large tumors), DEN-induced liver tumors (a mixture of five independent large tumors), CCl-induced cirrhotic liver tissues (non-tumorous tissues of livers harboring tumors, a mixture of five tissues from five mice), and control liver tissue (olive oil-treated; a mixture of two tissues from two mice) were analyzed and compared by one-color microarrays (3D-Gene Microarray; Toray, Tokyo, Japan).

Protocol tips
Samples of CCl-induced liver tumors (a mixture of five independent large tumors), DEN-induced liver tumors (a mixture of five independent large tumors), CCl-induced cirrhotic liver tissues (non-tumorous tissues of livers harboring tumors, a mixture of five tissues from five mice), and control liver tissue (olive oil-treated; a mixture of two tissues from two mice) were analyzed and compared by one-color microarrays (3D-Gene Microarray; Toray, Tokyo, Japan).

Protocol tips

Samples of CCl-induced liver tumors (a mixture of five independent large tumors), DEN-induced liver tumors (a mixture of five independent large tumors), CCl-induced cirrhotic liver tissues (non-tumorous tissues of livers harboring tumors, a mixture of five tissues from five mice), and control liver tissue (olive oil-treated; a mixture of two tissues from two mice) were analyzed and compared by one-color microarrays (3D-Gene Microarray; Toray, Tokyo, Japan).

Publication protocol 1 Relevant paper

Whole Mouse Genome Microarray Kit, 4x44K

Agilent Technologies

Upstream tips	Protocol tips	Downstream tips
Avoid touching the surface of the glass with your fingers. Skin oils and other substances, such as lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Upstream tips
Avoid touching the surface of the glass with your fingers. Skin oils and other substances, such as lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Manufacturer protocol Publication protocol 1 Relevant paper

Mouse Gene Expression v2 4x44K Microarray Kit

Agilent Technologies

Upstream tips	Protocol tips	Downstream tips
	Do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Protocol tips
Do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Manufacturer protocol Publication protocol 1 Relevant paper

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