Microarray Gene expression arrays - Rat chorid plexus Cyanine 3

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

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Whole Rat Genome Microarray Kit, 4x44K

Agilent Technologies

Upstream tips	Protocol tips	Downstream tips
Avoid touching the surface of the glass with your finersǯ Skin oils and other substances, such as lotions or ink, can fluoresceǯ If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Upstream tips
Avoid touching the surface of the glass with your finersǯ Skin oils and other substances, such as lotions or ink, can fluoresceǯ If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Manufacturer protocol Publication protocol 1 Relevant paper

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