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Found 1 matching solution for this experiment
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Microarray analysis was performed with Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays, using 250 ng RNA as input. All steps were performed according to the manufacturer’s instructions for the GeneChip® platform (3′IVT PLUS Reagent Kit, Affymetrix, Santa Clara, USA). The steps included first-strand cDNA synthesis, second-strand cDNA synthesis, synthesis of labeled cRNA by in vitro transcription, purification of labeled cRNA, fragmentation, array hybridization, washing, staining, and final scanning of the arrays using the GeneChip® Scanner 3000 7G. |
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| Protocol tips |
| Microarray analysis was performed with Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays, using 250 ng RNA as input. All steps were performed according to the manufacturer’s instructions for the GeneChip® platform (3′IVT PLUS Reagent Kit, Affymetrix, Santa Clara, USA). The steps included first-strand cDNA synthesis, second-strand cDNA synthesis, synthesis of labeled cRNA by in vitro transcription, purification of labeled cRNA, fragmentation, array hybridization, washing, staining, and final scanning of the arrays using the GeneChip® Scanner 3000 7G. |
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