Necrosis A549

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 3 matching solutions for this experiment

Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
• Kit contains recombinant annexin V conjugated to one of our best and brightest fluorophores, the Alexa Fluor® 488 dye, to provide maximum sensitivity. • Kit includes a ready-to-use solution of the red-fluorescent propidium iodide (PI) nucleic acid binding dye.	• The Alexa Fluor® 488 annexin V and propidium iodide are light sensitive and may be handled in normal room light, but avoid prolonged exposure to light. • Alexa Fluor® 488 annexin V: 488/499 in nm; • Propidium iodide: 535/617 in nm, bound to DNA.

Upstream tips
• Kit contains recombinant annexin V conjugated to one of our best and brightest fluorophores, the Alexa Fluor® 488 dye, to provide maximum sensitivity. • Kit includes a ready-to-use solution of the red-fluorescent propidium iodide (PI) nucleic acid binding dye.
Protocol tips
• The Alexa Fluor® 488 annexin V and propidium iodide are light sensitive and may be handled in normal room light, but avoid prolonged exposure to light. • Alexa Fluor® 488 annexin V: 488/499 in nm; • Propidium iodide: 535/617 in nm, bound to DNA.

Publication protocol 1 Relevant paper

RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay

Promega

Upstream tips	Protocol tips	Downstream tips
• No-wash, one-step Annexin V assay • Non-lytic. Allows continual monitoring of cell state to determine apoptotic onset • Scalable for high-throughput screening applications	• Seed 10,000 cells in 100μL/well on 96 white flat-bottomed multi-wells, and incubate overnight at 37◦C to allow adhesion.

Upstream tips
• No-wash, one-step Annexin V assay • Non-lytic. Allows continual monitoring of cell state to determine apoptotic onset • Scalable for high-throughput screening applications
Protocol tips
• Seed 10,000 cells in 100μL/well on 96 white flat-bottomed multi-wells, and incubate overnight at 37◦C to allow adhesion.

Manufacturer protocol Publication protocol 1 Relevant paper

GFP-CERTIFIED® Apoptosis/Necrosis detection kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
• Kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively.	• Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection

Upstream tips
• Kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively.
Protocol tips
• Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection

Manufacturer protocol Publication protocol 1 Relevant paper

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