Necrosis HeLa

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 3 matching solutions for this experiment

Upstream tips
• Kit contains recombinant annexin V conjugated to one of our best and brightest fluorophores, the Alexa Fluor® 488 dye, to provide maximum sensitivity.
• Kit includes a ready-to-use solution of the red-fluorescent propidium iodide (PI) nucleic acid binding dye.
Protocol tips
• The Alexa Fluor® 488 annexin V and propidium iodide are light sensitive and may be handled in normal room light, but avoid prolonged exposure to light.
• Alexa Fluor® 488 annexin V: 488/499 in nm;
• Propidium iodide: 535/617 in nm, bound to DNA.
Upstream tips
• Assay type: Cell-based
• Platform: Flow cytometer, Fluorescence microscope
• Assay time: 1 hr
• Sample type: Adherent cells, Suspension cells
Protocol tips
• 1×10^4/well in flat-bottom 96-well culture plates
Upstream tips
cKit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively.
Protocol tips
• Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection
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