No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
• A total number of 10,000 cells per sample were analyzed using a BD FACS Calibur flow cytometer (BD Biosciences, San Jose, CA, USA). |
• Wash cells with PBS, centrifuged and stained with Annexin V-FITC for 20 min at room temperature in the dark and subsequently stained with propidium iodide (PI) for 5 min before the measurement. |
• Fluorescence to be detected via a 530/30 nm band-pass filter (FL-1; Annexin V—FITC) and a 670 nm long-pass filter (FL-3; PI).
• Raw files can be analyzed using FlowJo software (Tree Star Inc., Ashland, OR, USA). |
Upstream tips |
• A total number of 10,000 cells per sample were analyzed using a BD FACS Calibur flow cytometer (BD Biosciences, San Jose, CA, USA). |
Protocol tips |
• Wash cells with PBS, centrifuged and stained with Annexin V-FITC for 20 min at room temperature in the dark and subsequently stained with propidium iodide (PI) for 5 min before the measurement. |
Downstream tips |
• Fluorescence to be detected via a 530/30 nm band-pass filter (FL-1; Annexin V—FITC) and a 670 nm long-pass filter (FL-3; PI).
• Raw files can be analyzed using FlowJo software (Tree Star Inc., Ashland, OR, USA). |
Acridine Orange solution + Ethidium bromide solution
Upstream tips |
Protocol tips |
Downstream tips |
• Dual Staining Procedure for differentiating Live/ Apoptotic/Necrotic cells |
• Seed 3x10^4 cells onto a 10mm coverslip and incubate overnight to form a confluent monolayer |
• The operator to be blinded to the experimental groups and random fields to be selected (40X objective).
• A total of 300 attached cells per cover-slip can be morphologically identified and counted as being either necrotic (red/orange nuclei),apoptotic (green condensed or fragmented nuclei) green or live (green non-condensed ovoid or rounded nuclei) |
Upstream tips |
• Dual Staining Procedure for differentiating Live/ Apoptotic/Necrotic cells |
Protocol tips |
• Seed 3x10^4 cells onto a 10mm coverslip and incubate overnight to form a confluent monolayer |
Downstream tips |
• The operator to be blinded to the experimental groups and random fields to be selected (40X objective).
• A total of 300 attached cells per cover-slip can be morphologically identified and counted as being either necrotic (red/orange nuclei),apoptotic (green condensed or fragmented nuclei) green or live (green non-condensed ovoid or rounded nuclei) |
Upstream tips |
Protocol tips |
Downstream tips |
• 2.5x10^4 cells/well were grown in 24-well plates |
|
• Fluorescence of cells was examined and quantified. |
Upstream tips |
• 2.5x10^4 cells/well were grown in 24-well plates |
Downstream tips |
• Fluorescence of cells was examined and quantified. |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!