Necrosis MIA PaCa-2

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 4 matching solutions for this experiment

Upstream tips
• Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.
• The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.
Protocol tips
• 5 X 10^5 cells were seeded in 6-well plates for 24 h
Upstream tips
• Detection Method Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
• Species Reactivity: Mammalian
• Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Protocol tips
• Seed 100,000 cells/mL in 6-well plates.
Upstream tips
• Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.
Protocol tips
• Seed 1 × 10^3 MiaCaPa-2 cells in each well.

Hoechst 33342 + Propidium Iodide - 1.0 mg/mL Solution in Water

Hoechst 33342

Thermo Fisher Scientific

Upstream tips
• Differential Staining for apoptosis and necrosis
Protocol tips
• Seed 15,000 cells/well
Downstream tips
•Apoptotic and necrotic cells were counted and the percentage number of cells was calculated.
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