Necrosis PANC-1

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 3 matching solutions for this experiment

Upstream tips
• Assay type: Cell-based
• Platform: Flow cytometer, Fluorescence microscope
• Assay time: 1 hr
• Sample type: Adherent cells, Suspension cells
Protocol tips
• 5 X 10^5 cells were seeded in 6-well plates for 24h
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