Necrosis T98-G

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 2 matching solutions for this experiment

Upstream tips
• Use untreated cells as negative and treat cells for 24 h with 1 μM docetaxel, an anticancer taxane drug, to use as positive control for apoptosis induction

Acridine Orange solution + Ethidium bromide solution

Upstream tips
• Five percent acridine orange was used to identify autophagous cells incubatedin the dark for 15 min. The dye induced the red glow of the autophagous bodies.
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