Found 2 discussions for this experiment
1 year ago
1 year ago by Maryam Amir
I keep getting a non-specific band in PCR so I would like to try a hot start PCR manually. How should I prepare the mix and at which step should I be adding the polymerase?
2 years ago
2 years ago by Janina Reinhardt
I am currently performing hot start PCR using phusion green hot start DNA polymerase in order to amplify a 8.8kb insert. However, I cannot get any amplified product at all. I have tried changing some parameters but I am not sure what to do. Any help?
Found 6 matching solutions for this experiment