PCR Multiplex PCR - Viral

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5 years ago

5 years ago by R. Verma India

Multiplex PCR and qPCR

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

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5 years ago

5 years ago by Roland Lafontaine France

My multiplex PCR has decreased in sensitivity

Hello there! My multiplex PCR has decreased in sensitivity even though I have not changed any of the parameters. The materials are in good condition and the PCR machine is well calibrated. I have tested multiple samples but one of my targets has decreased in sensitivity.

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Found 4 matching solutions for this experiment

QuantiTect Multiplex RT-PCR Kit (1000)

Qiagen

Protocol tips
Follow manufacturer’s instructions
Manufacturer protocol Publication protocol 1 Relevant paper
QuantiTect Multiplex RT-PCR NR Kit (1000)

Qiagen

Manufacturer protocol Publication protocol 1 Relevant paper
QuantiNova Multiplex RT-PCR Kit (2500)

Qiagen

Manufacturer protocol Publication protocol 1 Relevant paper
QuantiNova IC Probe Assay Red 650 (500)

Qiagen

Manufacturer protocol Publication protocol 1 Relevant paper
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