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Found 2 matching solutions for this experiment
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Recombinant P. pastoris clones were grown in YPD agar plates or RDB agar plates at 30°C. For expression studies, 2 ml of BMGY in culture plate (Dominique Dutscher, Brumath, France) were grown overnight at 30°C and 250 r.p.m. After 14–16 h, optical density was measured at 600 nm, and cells were resuspended in 2 ml of fresh BMMY medium to an initial OD600 of 1. |
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Protocol tips |
Recombinant P. pastoris clones were grown in YPD agar plates or RDB agar plates at 30°C. For expression studies, 2 ml of BMGY in culture plate (Dominique Dutscher, Brumath, France) were grown overnight at 30°C and 250 r.p.m. After 14–16 h, optical density was measured at 600 nm, and cells were resuspended in 2 ml of fresh BMMY medium to an initial OD600 of 1. |
Upstream tips |
Protocol tips |
Downstream tips |
|
Recombinant P. pastoris clones were grown in YPD agar plates or RDB agar plates at 30°C. For expression studies, 2 ml of BMGY in culture plate (Dominique Dutscher, Brumath, France) were grown overnight at 30°C and 250 r.p.m. After 14–16 h, optical density was measured at 600 nm, and cells were resuspended in 2 ml of fresh BMMY medium to an initial OD600 of 1. |
|
Protocol tips |
Recombinant P. pastoris clones were grown in YPD agar plates or RDB agar plates at 30°C. For expression studies, 2 ml of BMGY in culture plate (Dominique Dutscher, Brumath, France) were grown overnight at 30°C and 250 r.p.m. After 14–16 h, optical density was measured at 600 nm, and cells were resuspended in 2 ml of fresh BMMY medium to an initial OD600 of 1. |
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