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2 years ago
2 years ago by Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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|- Colonies are aseptically scraped off culture plates and resuspended in 500 μL distilled water.
- Bacterial cells are subsequently inactivated at 85°C for 30 minutes.
- Inactivated cells are centrifuged at 8,000 rpm for 5 minutes and the supernatant discarded.
|- Total bacterial protein was extracted following the manufacturer protocol. Instead of using 10mL of Native Lysis Buffer it is advised to use 1mL.|