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Found 3 matching solutions for this experiment
Upstream tips |
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- Instructions are followed regarding the manufacturer protocol, except fractions corresponding to cytosol, membrane and nuclei were boiled 5 min at 95°C in the presence of Laemmlli sample buffer. |
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Protocol tips |
- Instructions are followed regarding the manufacturer protocol, except fractions corresponding to cytosol, membrane and nuclei were boiled 5 min at 95°C in the presence of Laemmlli sample buffer. |
Upstream tips |
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- Cells are lysed using CelLytic MT mammalian tissue lysis/extraction reagent containing 1% complete protease inhibitor mixture according to the manufacturer’s protocol. |
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Protocol tips |
- Cells are lysed using CelLytic MT mammalian tissue lysis/extraction reagent containing 1% complete protease inhibitor mixture according to the manufacturer’s protocol. |
Upstream tips |
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After cell harvesting, the pellets were washed three times with cold PBS, lysed with RIPA buffer containing protease inhibitors, and sonicated using a sonicator |
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Protocol tips |
After cell harvesting, the pellets were washed three times with cold PBS, lysed with RIPA buffer containing protease inhibitors, and sonicated using a sonicator |
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