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Found 2 matching solutions for this experiment
|- Total proteins is extracted with Cell Lysis Buffer containing 1 mM phenylmethanesulfonyl fluoride (Sigma) on ice. The lysate is scraped and sonicated for 5 s by using an ultrasonic disruptor at output 1. The sonicate is centrifuged at 15,000 rpm for 10 min at 4°C.
|- For extraction follow the manufacturer's protocol.
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