Protein isolation Tissue - Human tissue C-MFPE samples

2 Matching solutions
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5 years ago

5 years ago by Jesualdo Hernández Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Found 2 matching solutions for this experiment

Qproteome Mammalian Protein Prep Kit

Qiagen

Upstream tips	Protocol tips	Downstream tips
	To ensure maximum protein extraction, first, heat the homogenized tissues at 90°C for 20 min followed by heating (1 h for tissues in mammalian tissue buffer) at 80°C.

Protocol tips
To ensure maximum protein extraction, first, heat the homogenized tissues at 90°C for 20 min followed by heating (1 h for tissues in mammalian tissue buffer) at 80°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Qproteome FFPE Tissue Kit (20)

Qiagen

Upstream tips	Protocol tips	Downstream tips
	To ensure maximum protein extraction, first, heat the homogenized tissues at 90°C for 20 min followed by heating (for 2 h for tissues in FFPE buffer) at 80°C.

Protocol tips
To ensure maximum protein extraction, first, heat the homogenized tissues at 90°C for 20 min followed by heating (for 2 h for tissues in FFPE buffer) at 80°C.

Manufacturer protocol Publication protocol 1 Relevant paper

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