Protein Ladder Prestained

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

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Found 51 matching solutions for this experiment

Upstream tips
Includes concentrated solutions of Chameleon 700 and Chameleon 800
1. The Chameleon 700 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 700 channel near-infrared detection.
Includes 7 protein bands from 8 to 260 kDa

2. The Chameleon 800 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 800 channel near-infrared detection.
Includes 6 protein bands from 8 to 260 kDa
Protocol tips
Includes 7 protein bands from 8 to 260 kDa
Includes 6 protein bands from 8 to 260 kDa
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For analysis use Image Studio 1.0-4.0
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Size range: 10-245 kDa
Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
Protocol tips
Do not heat, dilute, or add a reducing agent before loading.
Load 1.5 - 2.5µl per well for general Western transferring and 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
Upstream tips
Size range: 10 - 180 kDa
Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
Protocol tips
Do not heat, dilute, or add a reducing agent before loading.
Load 2 - 3 µl per well for general Western transferring and 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
Upstream tips
Size Range: 11 to 250 kDa
• Fluorescent visualization—detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager
• Western blot confirmation—detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein
Protocol tips
The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
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Size Range: 11 to 250 kDa
• Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers
• Reference band—55 kDa band has a greater intensity for easy orientation
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The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
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Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers
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Broad range: 10 – 180 kDa
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Recommendations for Loading
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

5 μl per well for mini-gels, 3 μl per well for blots
10 μl per well for large gels, 6 μl per well for blots
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Molecular Weight Marker Ranges from: 1.7-40 kDa
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1.) Thaw the ladder either at Room Temp. or at 37°C for a few minutes to dissolve precipitated solids thoroughly. DO NOT BOIL.
2.) Mix gently, but thoroughly to ensure that the solution is homogeneous.
3.) Load following volumes of the ladder on
Min SDS-PAGE gel: 5ul per well, with a thickness of 0.75-1.0mm
Large SDS_Polyacrylamide gel: 10ul per well, with a thickness of 0.75-1.0mm.
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The broad molecular weight ranges from - 1o to 250 kDa
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Do Not Boil Protein Marker
1. Thaw the protein ladder on ice.
2. Gently vortex solution to ensure the mixture is homogeneous.
3. Load the appropriate volume of the protein marker per lane, as specified below:

Mini-Gel:
0.75-1.0 mm thick: load 5 μl
1.5 mm thick: load 10 μl

Large Gel:
0.75-1.0 mm thick: load 10μl
1.5 mm thick: load 20 μl
Upstream tips
The broad molecular weight ranges from - 11-250 kDa
Protocol tips
Do Not Boil Protein Marker
1. Thaw the protein ladder on ice.
2. Gently vortex solution to ensure the mixture is homogeneous.
3. Load the appropriate volume of the protein marker per lane, as specified below:

Mini-Gel:
0.75-1.0 mm thick: load 5 μl
1.5 mm thick: load 10 μl

Large Gel:
0.75-1.0 mm thick: load 10μl
1.5 mm thick: load 20 μl
Upstream tips
Molecular weight ranges from - 43 to 315 kDa
Protocol tips
Do Not Boil Protein Marker
1. Thaw the protein ladder on ice.
2. Gently vortex solution to ensure the mixture is homogeneous.
3. Load the appropriate volume of the protein marker per lane, as specified below:

Mini-Gel:
0.75-1.0 mm thick: load 5 μl
1.5 mm thick: load 10 μl

Large Gel:
0.75-1.0 mm thick: load 10μl
1.5 mm thick: load 20 μl
Upstream tips
Molecular weight ranges from - 1.7 to 42 kDa
Protocol tips
Do Not Boil Protein Marker
1. Thaw the protein ladder on ice.
2. Gently vortex solution to ensure the mixture is homogeneous.
3. Load the appropriate volume of the protein marker per lane, as specified below:

Mini-Gel:
0.75-1.0 mm thick: load 5 μl
1.5 mm thick: load 10 μl

Large Gel:
0.75-1.0 mm thick: load 10μl
1.5 mm thick: load 20 μl
Upstream tips
Molecular weight ranges from: 11-250 kDa
Protocol tips
Do Not Boil Protein Marker
1. Thaw the protein ladder on ice.
2. Gently vortex solution to ensure the mixture is homogeneous.
3. Load the appropriate volume of the protein marker per lane, as specified below:

Mini-Gel:
0.75-1.0 mm thick: load 5 μl
1.5 mm thick: load 10 μl

Large Gel:
0.75-1.0 mm thick: load 10μl
1.5 mm thick: load 20 μl
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Size Range: 20 to 120 kDa
Component proteins: beta-galactosidase (120 kDa), bovine serum albumin (85 kDa), ovalbumin (50 kDa), cardonic anhydrase (35 kDa), beta-lactoglobulin (25 kDa), and lysozyme (20 kDa)
Protocol tips
The protein standards are supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
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Detection Method: Colorimetric
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Molecular weight ranges from 1.7 to 40 kDa
Protocol tips
Load an appropriate volume of the ladder on the gel.
• Mini-gel: 5µL per well (0.75-1.0mm thick) or 10µL per well (1.5mm thick)
• Midi gel: 10µL per well (0.75-1.0mm thick) or 20µL per well (1.5mm thick)
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Detection Method:
colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel)
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Size range: 6.5-270 kDa
Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Upstream tips
Size range: 3.5-245 kDa
Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
Protocol tips
Do not heat, dilute, or add a reducing agent before loading.
Recommended loading: ~1.5 - 2.5µl
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Molecular weight ranges from 3.5 to 245 kDa
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• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,
respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (>1.5 mm) or larger gel.
Upstream tips
Molecular Weight ranges from 11-245 kDa
Protocol tips
• 3 µl or 5 µl per loading for clear visualization during electrophoresis
• 1.5-2.5 µl per well for general Western transferring on 15-well or 10-well
mini-gel, respectively.
• Apply more for thicker (>1.5 mm) or larger gel.
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When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa
Protocol tips
This ladder is pre-mixed with loading buffer and ready to load. Pre-heating
is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel
for standard use
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Size Range: 10 to 180 kDa
Protocol tips
The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
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Detection Method
colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel)
Upstream tips
Pre-stained proteins with molecular weights ranging from 3 to 260 kDa
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
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1. Uniquely tailor-made Prestained or Unstained Protein Ladder according to one’s specifications.

2. Minimized Entry Volume Requirement – 500ml to 10 liters, with 3 to 12 protein bands and 1 to 3 color selections
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Molecular weights ranges from 10 to 180 kDa
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3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
2.5 μl per well for general Western transferring.
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Molecular weight ranges from 6.5 to 270 kDa
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring
Upstream tips
Molecular weight ranges from 11 to 245 kDa
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Upstream tips
Molecular weight ranges from 15 to 180 kDa
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring
Upstream tips
Molecular weight ranges from 11 to 180 kDa
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring
Upstream tips
Molecular weight ranges from 15 to 170 kDa
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3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring
Upstream tips
Molecular Weight ranges from 5 to 245 kDa
Protocol tips
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Upstream tips
Size Range: 6 to 180 kDa
Protocol tips
The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Downstream tips
Detection Method: Colorimetric
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Molecular weights range from: 6.5 to 270 kDa
Protocol tips
5ul of BLUltra Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to the nitrocellulose membrane.
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Molecular weight ranges from 10 to 180 kDa
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5ul of BlueAQUA Prestained Protein Ladder resolves 11 bands in 4-15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to a nitrocellulose membrane.
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Molecular Weight Ranges from: 5 to 245 kDa
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5ul of BLUelf Prestained Protein Ladder resolves 13 bands in 4-15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to a nitrocellulose membrane.
Upstream tips
Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly.
Protocol tips
Includes 11 protein bands from 8 to 260 kDa
Downstream tips
For analysis use Image Studio 1.0-4.0
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Molecular Weight Ranges from: 25 kDa and 75 kDa
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5ul of BlueRAY Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
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The ready-to-use BLUeye prestained protein ladder has 12 sharp bands at 11, 17, 20, 25, 35,48, 63, 75, 100, 135, 180, and 245 kDa, which has more bands than commonly used markers.
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MW size range: varies by standard
Natural Protein Standard ranges from: Myosin, β-Galactosidase, BSA, Carbonic anhydrase, Soybean trypsin inhibitor, Lysozyme to Aprotinin
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Size Range: 10-180 kDa
It includes a red reference band at 75 kDa and a green reference band at 25 kDa.
Protocol tips
For loading use assay dependent concentration for
WB and SDS-PAGE
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Broad range: 6.5 – 270 kDa
Protocol tips
3 μl or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
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Molecular weight range: 10-250 kDa
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Gently mix the Color Prestained Protein Standard, Broad Range (10-250 kDa).
Load 3 μl directly onto a mini-gel.
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Molecular weight range: 10 – 180 kDa
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We recommend 4-5 µl per well for protein electrophoresis and 2-3 µl per well for Western Transfer
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Molecular weight ranges from 40 kDa to 300 kDa
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Detection Method:
colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel)
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Ranges from 11-250 kDa
Protocol tips
Direct loading, additional loading buffer and heat incubation not required
Load 3 μl directly onto a mini-gel
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Size Range: 10-180 kDa
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Load: 3- 5 µl/ gel well for electrophoresis
Load: 2- 3 µl/ gel well for Western transfers
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Size Range: 11 - 310 kDa
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Load: 3- 5 µl/ gel well for electrophoresis
Load: 3- 5 µl/ gel well for Western transfers
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Size Range: 3.5-245 kDa
Protocol tips
Load: 3- 5 µl/ gel well for electrophoresis
Load: 2- 3 µl/ gel well for Western transfers
Prestained Protein Standards

Bio-Rad Laboratories

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Molecular Masses ranges from 10 to 250 kD
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Recommended loading volume:
Electrophoresis: 10 μl
Blotting: 7 μl
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Molecular weight ranges from: 3.5 - 245 kDa
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Recommended loading: 3 µL or 5 µL for clear visualization
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Molecular Weight ranges from 10-180 kDa
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Recommended Load: 3-5 μl Protein Molecular Weight Marker per lane
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Molecular weight ranges from 5-245 kDa
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Recommended Load: 3-5 μl Protein Molecular Weight Marker per lane
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Wide range of molecular weights from 10 to 245 kDa
Protocol tips
Recommended load: 5 µl/lane
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