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Found 5 matching solutions for this experiment
|Human iPS cell lysates were prepared by dissolving Tic cells (1.25 mg protein/1 × 107 cells, as determined with a Micro BCA protein assay kit (Pierce-Thermo Scientific)) in the complete RIPA buffer (0.5 mL) under sonication.
|Total protein was extracted from the human cervical cancer samples and non-cancer samples. 2-D Quant kits (Amersham Biosciences, Little Chalfont, UK) were used to determine the total concentrations.
|The protein concentration of cell lysates was determined by bicinchoninic acid (BCA) assay (QuantiPro BCA Assay Kit, Sigma-Aldrich) according to the manufacturer’s instructions.
|Protein concentrations were determined using the BCA assay (Pierce) using bovine serum albumin (BSA) as standard.
|Protein concentration was determined using Bicinchoninic Acid Protein Assay (Pierce™, Rockford, IL, USA).
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