Reporter gene assay β-galactosidase substrates - adipose stem cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 4 matching solutions for this experiment

Senescence β-Galactosidase Staining Kit - Cell Signaling

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
Seed 3 × 10 cells per well.	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Upstream tips
Seed 3 × 10 cells per well.
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence Cells Histochemical Staining Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.	Measure for absorbance at 420 nm

Protocol tips
Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.
Downstream tips
Measure for absorbance at 420 nm

Manufacturer protocol Publication protocol 1 Relevant paper

Cellular Senescence Staining Kit

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	fix cells with fixing solution at RT for 5 min. Wash cells and incubate in working solution for 4 h at 37 °C

Protocol tips
fix cells with fixing solution at RT for 5 min. Wash cells and incubate in working solution for 4 h at 37 °C

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence β-Galactosidase Staining Kit - Beyotime

Beyotime

Upstream tips	Protocol tips	Downstream tips
Seed 5 × 10 hASCs cells	Incubate overnight at 37 ° C without Co2

Upstream tips
Seed 5 × 10 hASCs cells
Protocol tips
Incubate overnight at 37 ° C without Co2

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox