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Found 4 matching solutions for this experiment
|Seed 3 × 10 cells per well.
|Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
|Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.
|Measure for absorbance at 420 nm
|fix cells with fixing solution at RT for 5 min.
Wash cells and incubate in working solution for 4 h at 37 °C
|Seed 5 × 10 hASCs cells
|Incubate overnight at 37 ° C without Co2
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