Reporter gene assay β-galactosidase substrates - adipose stem cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 4 matching solutions for this experiment

Upstream tips
Seed 3 × 10 cells per well.
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
Protocol tips
Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.
Downstream tips
Measure for absorbance at 420 nm
Protocol tips
fix cells with fixing solution at RT for 5 min.

Wash cells and incubate in working solution for 4 h at 37 °C
Upstream tips
Seed 5 × 10 hASCs cells
Protocol tips
Incubate overnight at 37 ° C without Co2
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