Reporter gene assay β-galactosidase substrates - Bxpc-3

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Upstream tips
Seed cells fro 72 hours and washe twice with PBS,

Fix for 6 min with fixation buffer containing 2% formaldehyde, 0.2% glutaraldehyde, 7.0 mM Na2HPO4, 1.5 mM KH2PO4, 140 mM NaCl, and 2.68 mM KCl
Protocol tips
Add 1 ml of the Staining Mixture per well and incubate overnight at 37 °C without CO2 until the cells are stained blue.
Protocol tips
Fix the cells with 0.5 ml of Fixative Solution for 10 - 15 min at room temperature

Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.
Upstream tips
Seed 5 × 10^4 cells
Protocol tips
Add 100µL of β-Galactosidase Assay Reagent.

Cover plate and incubate for 30 minutes at 37°C.
Downstream tips
Measure absorbance at 405nm.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms