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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
Seed 1 × 10^5 cells per well |
Add 150µl of Assay 2X Buffer to samples.
Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed
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Read the absorbance at 420nm |
Upstream tips |
Seed 1 × 10^5 cells per well |
Protocol tips |
Add 150µl of Assay 2X Buffer to samples.
Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed
|
Downstream tips |
Read the absorbance at 420nm |
Upstream tips |
Protocol tips |
Downstream tips |
Fix cells after 72 hrs of miR-7 transfection. |
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2) |
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Upstream tips |
Fix cells after 72 hrs of miR-7 transfection. |
Protocol tips |
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2) |
Upstream tips |
Protocol tips |
Downstream tips |
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Dilute Galacton-Plus substrate 1:100 with reaction buffer diluent to make a reaction buffer.
Add 70 ul of this buffer and incubate for 30-60 min |
|
Protocol tips |
Dilute Galacton-Plus substrate 1:100 with reaction buffer diluent to make a reaction buffer.
Add 70 ul of this buffer and incubate for 30-60 min |
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