Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 2 matching solutions for this experiment

Senescence β-Galactosidase Staining Kit - Cell Signaling

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
	Fix cells with 0.5 mL 1Х fixative solution for 15 min at room temperature. Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)	Visualise cells after 11 to 14 hr,

Protocol tips
Fix cells with 0.5 mL 1Х fixative solution for 15 min at room temperature. Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
Downstream tips
Visualise cells after 11 to 14 hr,

Manufacturer protocol Publication protocol 1 Relevant paper

Luminescent β-galactosidase Detection Kit II

Takara Bio Inc

Upstream tips	Protocol tips	Downstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature	Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently. Incubate at room temperature (20–25°C) for 60 min

Upstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature
Protocol tips
Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently. Incubate at room temperature (20–25°C) for 60 min

Manufacturer protocol Publication protocol 1 Relevant paper

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