Reporter gene assay β-galactosidase substrates - MIA PaCa-2

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

β-Gal Reporter Gene Assay, chemiluminescent

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Wash cells and add β-Gal Reporter Gene Assay, incubate for 30 min at RT. Add substrate reagent and incubate for 15-60 min at RT

Protocol tips
Wash cells and add β-Gal Reporter Gene Assay, incubate for 30 min at RT. Add substrate reagent and incubate for 15-60 min at RT

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence β-Galactosidase Staining Kit - Cell Signaling

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C for 48 hours in a dry incubator (no CO2)

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C for 48 hours in a dry incubator (no CO2)

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox