Reporter gene assay β-galactosidase substrates - mouse embryo tissue

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 1 matching solution for this experiment

β-Galactosidase Reporter Gene Staining Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Analyze the β-galactosidase expression 40-72 hr Add lysis buffer and incubate at room temperature for 15 minutes. Add 2x assay buffer and incubate at 37 °C for 2h or until a yellow color develops (t min).	Read OD at 420 nm

Protocol tips
Analyze the β-galactosidase expression 40-72 hr Add lysis buffer and incubate at room temperature for 15 minutes. Add 2x assay buffer and incubate at 37 °C for 2h or until a yellow color develops (t min).
Downstream tips
Read OD at 420 nm

Manufacturer protocol Publication protocol 1 Relevant paper

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