Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

Upstream tips
Pretreat cells with TM5441 for 24 h followed by Doxorubicin (50 nM) treatment in triplicate for 4 days
Protocol tips
Fix cells with fixative solution for 15 min at room temperature.

Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.
Protocol tips
Fix cells for 7 min in 1×fixative solution.

Add 1 ml of the Staining Mixture per well and incubate for 48 h at 37 °C without CO2 until the cells are stained blue
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
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