Reporter gene assay β-galactosidase substrates - RAW 264.7

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

Upstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature
Protocol tips
Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently.

Incubate at room temperature (20–25°C) for 60 min
Protocol tips
Add diluted Galacton-Plus substrate 1:100 with Reaction Buffer Diluent.

Add 70µL of Reaction Buffer, mix, and incubate for 30-60 min
Protocol tips
Wash cells and add β-Gal Reporter Gene Assay, incubate for 30 min at RT.

Add substrate reagent and incubate for 15-60 min at RT
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