Reporter gene assay β-galactosidase substrates - U87 and U251 glioblastoma cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 1 matching solution for this experiment

Protocol tips
Add diluted Galacton-Plus substrate 1:100 with Reaction Buffer Diluent.

Add 70µL of Reaction Buffer, mix, and incubate for 30-60 min
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