Reporter gene assay β-lactamase substrates - Burkholderia cepacia complex

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Downstream tips
Measure the absorbance (A490) in a microplate
reader in kinetic mode for 30–60 minutes at room
temperature.
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