Reporter gene assay luciferase - BHK-21 baby hamster kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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4 years ago

4 years ago by J.Han China

Which transfection reagent would work better on stem cells?

I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?

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Found 1 matching solution for this experiment

Upstream tips
10^4 cells into 96-well plates for 24 hr
Protocol tips
Transfecte with 0.2 μg Tax expression plasmid.

Performed luciferase by using One Glo system assay after 48 h of transfection.

Add 100µl of reagent to the cells grown in 100µl of medium.

Downstream tips
Wait at least 3 minutes to allow complete cell lysis and measure in a luminometer
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