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2 years ago
2 years ago by J.Han
I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?
Found 1 matching solution for this experiment
|10^4 cells into 96-well plates for 24 hr|
|Transfecte with 0.2 μg Tax expression plasmid.
Performed luciferase by using One Glo system assay after 48 h of transfection.
Add 100µl of reagent to the cells grown in 100µl of medium.
|Wait at least 3 minutes to allow complete cell lysis and measure in a luminometer|