RNA isolation / purification Cells - immortalized 293T

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Start discussion

Found 1 discussion for this experiment

Discussion

2 years ago

2 years ago by Ralf Friedmann Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Found 1 matching solution for this experiment

RNAzol® RT

Molecular Research Center, Inc.

Upstream tips
To reduce RNA degradation do not wash cells before the addition of RNAzol® RT.
Protocol tips
To reduce DNA contamination, extend the incubation time to 15 minutes before sedimentation.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms