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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.
- Addition of β-mercapto ethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation.
- To capture more amount of mRNA, modify the amount of lysis buffer:ethanol (from 1:1 to 1:1.5) during the binding step. |
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA.
- Digest with 100 μg/mL Proteinase K in the presence of 0.5% SDS at 37°C for 30 min. |
Protocol tips |
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.
- Addition of β-mercapto ethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation.
- To capture more amount of mRNA, modify the amount of lysis buffer:ethanol (from 1:1 to 1:1.5) during the binding step. |
Downstream tips |
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA.
- Digest with 100 μg/mL Proteinase K in the presence of 0.5% SDS at 37°C for 30 min. |
Upstream tips |
Protocol tips |
Downstream tips |
|
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step |
|
Protocol tips |
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step |
Upstream tips |
Protocol tips |
Downstream tips |
|
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
|
Protocol tips |
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
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