Found 1 discussion for this experiment
1 year ago
1 year ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Found 3 matching solutions for this experiment
|- This kit is designed to process upto 2x10^6 cells.
- Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.
|- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step.
- If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit.
|- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use.
- If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.