Found 1 discussion for this experiment
4 years ago
4 years ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Found 2 matching solutions for this experiment
|- As COS cells have high RNA content do not use more than 3 x 10*6 cells as a starting material, otherwise the RNA binding capacity of the RNeasy spin column will be exceeded and cause low yield.
|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
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