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1 year ago
1 year ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
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|Total RNA was extracted using the RNAqueous™ Micro Kit (Life Technologies, USA) for nucleic acid extraction from small cell populations. All cell populations of interest were lysed directly on the membranes without prior enzymatic detachment. Isolated RNA was immediately tested on yield, purity and quality using a NanoDrop® ND-1000 (Thermo Scientific, USA) and a BioAnalyzer 2100 (Agilent Technologies, USA) device. Samples were stored at -80°C and only samples with absorption (A) ratios A260/A280 ≥ 1.8 and A260/A230 ≥ 1.5 (NanoDrop®) and RNA Integrity Number (RIN) > 6 (BioAnalyzer) were considered for microarray hybridization.|