RNA isolation / purification Cells - primary human keratinocytes

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

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2 years ago

2 years ago by Ralf Friedmann Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Protocol tips
Total RNA was purified using RNeasy Mini Kits (Qiagen Inc., Valencia, CA). RNA samples were treated with DNase I (Qiagen, Inc.) prior to synthesis of cDNA using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen/Thermo Fisher Scientific).
Protocol tips
Total RNA was extracted by miRNeasy kit (Qiagen) from both tissue samples and cells. RNA concentrations were measured by Nanodrop and Qubit and the quality was controlled by Bioanalyzer (RIN for all samples >8; Additional file 2: Table S2).
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