Found 2 discussions for this experiment
2 years ago
2 years ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
3 years ago
3 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 1 matching solution for this experiment
|- To reduce DNA contamination wash pellet with 0.1 M sodium citrate in 10% ethanol.
- To increase RNA yield, Increase the solubilization by pipetting the sample repeatedly, heat the sample to 60 °C
|- If there is Low A260/280 for RNA,ensure that 1 mL TRIsure per 10 cm2 area of cells or 5 x 10^6 cells is used.