Found 2 discussions for this experiment
3 years ago
3 years ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
4 years ago
4 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 3 matching solutions for this experiment
|- The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit.
- Do not use more sample than this kit is designed to handle.
- Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas.
|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).|
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
|- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.
|- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation
|- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results
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