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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
- To allow complete penetration by formalin, use tissue samples less than 5 mm thick
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- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C
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- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay
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Upstream tips |
- To allow complete penetration by formalin, use tissue samples less than 5 mm thick
|
Protocol tips |
- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C
|
Downstream tips |
- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay
|
Upstream tips |
Protocol tips |
Downstream tips |
- Aliquot rDNase and store at -20 °C.
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Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.
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Upstream tips |
- Aliquot rDNase and store at -20 °C.
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Protocol tips |
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.
|
Upstream tips |
Protocol tips |
Downstream tips |
- Can be used when there is a very less amount of starting material
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Upstream tips |
- Can be used when there is a very less amount of starting material
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