Found 2 discussions for this experiment
4 years ago
4 years ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
4 years ago
4 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 3 matching solutions for this experiment
|- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.
|- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation
|- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results
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