Found 2 discussions for this experiment
1 year ago
1 year ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
2 years ago
2 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 2 matching solutions for this experiment
|- This kit purifies RNA from up to 30 mg tissue|
|- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging.
- If there is low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity.
- If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.